Effect of desiccation on somatic embryos

Desiccation is necessary to normalize subsequent germination and development from soybean somatic embryos. The embryos below are of the same age and genotype. Those on the right were desiccated for 7 days, while those on the left were not:

For more information, see:

N. Hammatt and M. R. Davey. 1987. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J.Plant Physiol. 128:219-226.

W. A. Parrott, G. Dryden, S. Vogt, D. F. Hildebrand, G. B. Collins, and E. G. Williams. 1988. Optimization of somatic embryogenesis and embryo germination in soybean. In Vitro Cell.Dev.Biol. 24:817-820.

J. A. Buchheim, S. M. Colburn, and J. P. Ranch. 1989. Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol. 89:768-775.