Genome editing vectors
Last modified October 2, 2014
REFERENCE:
Jacobs, TB, PR LaFayette, MA Ortega, RJ Schmitz, WA Parrott. 2015. Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 15:16. PDF
What is our CRISPR vector family?
The CRISPR family is a series of binary vectors for plant transformation. The selectable markers include GFP, bar, hygromycin, and nptII. Additional guide RNAs can be introduced with the meganuclease I-PpoI.
In our hairy-root system, >95% of events contain modifications and >70% of alleles are modified.
Knockout of GFP with CRISPR in soybean:
Protocols for CRISPR vectors:
Where can I obtain the CRISPR family vectors?
Click the vector maps to download the vector (.gb) files
