University of Georgia

A website from the College of Agricultural and Environmental Sciences

Parrott Lab

Arabitol dehydrogenase

A non-antibiotic marker for the selection of transgenic plants
Last modified 4 November 2006

  • E. coli strain C and other related bacteria have the ability to use arabitol as the sole carbon source. The photo at left shows E. coli strains K12 (top row) and C (bottom row) growing on minimal medium with arabitol (left column) and LB (right column).
  • The gene was isolated from E. coli C. The wildtype sequence is in GenBank as AF359520
  • Photo at left shows a rice callus growing on arabitol-supplemented medium Best appears to be 0.25% sucrose and 2.75% arabitol
  • Transformation frequencies are equivalent to those obtained with hygromycin as the selectable marker
  • Germination of rice seedlings on medium supplemented with arabitol Left: Nontransgenic
  • Right: T1 rice seedlings transgenic for atlD

FAQs:

What is the reference for atlD?

  • LaFayette, P.R., P.M. Kane, B.H. Phan, and W.A. Parrott. 2005. Arabitol dehydrogenase as a selectable marker for rice. Plant Cell Rep. 24:596 – 602. PDF.

Are there any restrictions on the use of atlD?

  • The synthetic atlD is made available on a non-exclusive basis for non-commercial research uses, with the following conditions:
    • This gene will not be transferred to any third parties without permission from their originators. Any publications resulting from the use of this gene will acknowledge their use and cite the appropriate reference No guarantees, explicit or implied, on the effectiveness or efficiency of the gene, are made by their originators or The University of Georgia, nor can their originators or The University of Georgia be held liable for failure of the gene to perform as expected, or any consequences resulting therefrom.
    • Anyone desiring to use this gene for any commercial purpose must contact Shelley Fincher at The University of Georgia Technology Transfer Office for additional information.