FNLite Salt Formulation

Per liter of medium:

• Macro Salts: A 10x stock solution may be made of the following 4 ingredients:
  1. (NH₄)₂SO₄ – 463 mg
  2. KNO₃ – 2830 mg
  3. MgSO₄·7H₂O – 370 mg
  4. KH₂PO₄ – 185 mg
• Ca is reduced from the standard 2.99 mM in MS and FN media to 2.04 mM [= 300 mg/L]
• Iron as per Murashige & Skoog

For more information, see:

Samoylov, V.M., D.M. Tucker, and W.A. Parrott. 1998. Soybean [Glycine max (L.) Merrill] embryogenic cultures: the role of sucrose and total nitrogen content on proliferation. In Vitro Cell Dev.Biol.- Plant 34:8-13.